| distance | reference number | name | latitude | longitude |
| 1.0 | 50 | Amelia City, South Amelia River, Florida | 30.5867 | -81.4633 |
| 2.6 | 1520 | Kingsley Creek, RR. bridge, Florida | 30.6317 | -81.4767 |
| 4.3 | 1948 | Nassauville, St. Marys River, Florida | 30.5683 | -81.515 |
| 4.5 | 973 | Fernandina Beach, Amelia River, Florida | 30.6717 | -81.465 |
| 4.8 | 2929 | St. Marys River (entrance), Florida | 30.5183 | -81.4533 |
| 5.1 | 2692 | Sawpit Creek entrance, bridge, Florida | 30.5133 | -81.4567 |
| 5.2 | 1667 | Lofton, Lanceford Creek, St. Marys River, Florida | 30.6433 | -81.5233 |
| 5.8 | 2693 | Sawpit Creek, 1 mi. above entrance, Florida | 30.5033 | -81.4717 |
| 6.4 | 3093 | Tiger Point, Pumpkin Hill Creek, St. Marys River, F | 30.5017 | -81.495 |
| 7.2 | 545 | Chester, Bells River, St. Marys River, Florida | 30.6833 | -81.5333 |
| 7.2 | 2928 | St. Marys Entrance, North Jetty, Georgia | 30.7167 | -81.4333 |
| 7.4 | 713 | Cuno, Lofton Creek, St. Marys River, Florida | 30.5767 | -81.5717 |
| 7.9 | 192 | Beach Creek ent., Cumberland Island, Georgia | 30.7267 | -81.4767 |
| 8.0 | 886 | Edwards Creek, 1 mi. above entrance, Florida | 30.5017 | -81.5417 |
| 8.1 | 2802 | Simpson Creek, A1A highway bridge, Florida | 30.465 | -81.4317 |
| 8.7 | 1848 | Mink Creek entrance, St. Marys River, Florida | 30.5367 | -81.5817 |
| 9.4 | 2930 | St. Marys, St. Marys River, Florida | 30.72 | -81.5483 |
| 9.5 | 1022 | Fort George Island, Fort George River, Florida | 30.44 | -81.4383 |
| 9.6 | 1198 | Halfmoon Island, highway bridge, St. Marys River, F | 30.575 | -81.6083 |
| 9.9 | 2590 | Roses Bluff, Bells River, St. Marys River, Florida | 30.7033 | -81.5767 |
| 10.0 | 2716 | Seacamp Dock, Cumberland Island, Georgia | 30.7633 | -81.4717 |
| 10.4 | 1657 | Little Talbot Island, Florida | 30.43 | -81.405 |
| 10.9 | 2804 | Sisters Creek, St. Johns River, Florida | 30.4167 | -81.4533 |
| 12.0 | 577 | Clapboard Creek, Pelotes Island, St. Johns River, F | 30.4067 | -81.51 |
| 12.1 | 1793 | Mayport Naval Station, Florida | 30.4 | -81.4133 |